Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Size:1000g 2 at 25°C Suspend 50

2 at 25°C Suspend 50 gram powdered medium in 1 litre distilled water Store dry at room temperature

Malt Extract 30 Adenine 0

0 Beef Extract 5

Luria Broth Base or L-Broth is based upon the Luria Broth formulation as originally developed by Miller for cultivation and maintenance of E

2nd edition ed

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Size:1000g 2 at 25°C Suspend 50Calcium chloride, CaCl2 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium Nitrate. Suspend 1. 8 gram powdered medium in 1 litre distilled water Store dry at room